FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213303
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCCT346300.021.5109186
TCATCCC346150.021.5043495
ATCCCTT347050.021.4962737
CCTTCCC346350.021.4787810
TCATTCA348950.021.4725841
GGAGCAG86100.021.4507264
CCCTTCC348450.021.4345689
TTCATCC347450.021.4270534
CATTCAT349550.021.3374232
ATTCATC350400.021.2466623
GAGGAAG78950.021.1362483
CGGGAGG45250.021.0763284
GGCGTAG45800.021.0393893
CATCGGG91550.021.0168721
ATGGGAG48850.020.9888171
AGGAGCA59700.020.968263
GGGAGGA45400.020.9582355
TCCCTTC357050.020.9121278
TCGGGAG45600.020.8904363
TAGGAGC59750.020.8783762
Produced by FastQC (version 0.11.5)