FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8274245
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCCC396000.021.5198635
CATCCCT397100.021.4717226
ATCCCTT397550.021.4715427
TTCATCC397750.021.4473084
CCTTCCC397300.021.4267810
TCATTCA400650.021.4235691
CCCTTCC399500.021.4106649
CATTCAT400600.021.3319682
ATTCATC400550.021.3166073
CGGGAGG50800.021.2425384
GGAGCAG97050.021.2296774
GGAAAGA51000.021.223439
GAGGAAG93450.021.1999863
AGGAGCA66600.021.0424523
AGGAAGC54400.020.989384
CGATCAC101050.020.9880394
ATGGGAG57750.020.9742451
CATCGGG104400.020.9702511
TCCCTTC408150.020.964868
AGCCGAG44300.020.957523
Produced by FastQC (version 0.11.5)