FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1-1_202009_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1-1_202009_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15588188
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATCC752350.021.4930634
TCATCCC751100.021.492225
GGAGCAG183300.021.4723114
TCATTCA757900.021.4680921
CATCCCT752100.021.4651766
ATCCCTT752600.021.4548857
CCTTCCC750150.021.45415910
CCCTTCC754550.021.4179789
CATTCAT758950.021.3436222
ATTCATC758200.021.3301323
CGGGAGG101200.021.2177944
GAGGAAG165050.021.193983
GGGAGGA101550.021.1121675
TAGGAGC128050.021.039492
GGAACCG87550.021.0341662
AGGAGCA128200.021.0136593
TCGGGAG101600.021.0043353
CATCGGG205250.020.9945981
TCCCTTC772050.020.952388
AGCCGAG84650.020.9218373
Produced by FastQC (version 0.11.5)