FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1-1_202009_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1-1_202009_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18351775
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCCC883900.021.5299825
TCATTCA893850.021.4907971
CCTTCCC885200.021.47280710
CATCCCT886850.021.4611976
TTCATCC888750.021.4347114
ATCCCTT888400.021.4316027
CCCTTCC892450.021.3883449
CATTCAT894600.021.3588282
GGAGCAG210750.021.3479024
ATTCATC895750.021.302823
GAGGAAG197000.021.2241923
CGGGAGG117600.021.074314
AGCCGAG100300.020.9913583
ATGGGAG121300.020.9688721
CATCGGG234500.020.9472311
GGGAGGA118350.020.9315225
GGAAAGA118200.020.9201769
AGGAAGC116350.020.9130924
TCCCTTC913050.020.9093998
TACTGAC175400.020.8833969
Produced by FastQC (version 0.11.5)