FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1-1_202009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1-1_202009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20135087
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCCT970800.021.54276
TCATCCC971300.021.5338765
TCATTCA982650.021.5052591
ATCCCTT974550.021.4830287
CCTTCCC973850.021.43597610
TTCATCC976800.021.426144
CCCTTCC979550.021.411189
GGAGCAG238700.021.3414784
ATTCATC983350.021.3326423
CATTCAT984800.021.326922
GAGGAAG212400.021.1562963
AGGAGCA166000.021.0661183
GGTAATC246250.021.0596981
CGGGAGG127650.021.0353164
CATCGGG263400.021.0041281
ATGGGAG134900.020.9666421
TCCCTTC1002850.020.9278728
GGCGTAG121550.020.905433
GGAACCG116350.020.9048522
AGGAAGC121950.020.881964
Produced by FastQC (version 0.11.5)