FastQCFastQC Report
Wed 30 Sep 2020
HVCVGBGXG_n01_CRISPR_12hpf1-1_202009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCVGBGXG_n01_CRISPR_12hpf1-1_202009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15862649
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATCC774150.021.494814
TCATCCC773300.021.4928345
CATCCCT773500.021.4887666
CCTTCCC771600.021.47915810
ATCCCTT775100.021.4546787
TCATTCA782450.021.4428881
CCCTTCC777050.021.4289479
GGAGCAG187150.021.4070114
CATTCAT780200.021.3811762
ATTCATC780950.021.3245533
GAGGAAG169250.021.2987293
CGGGAGG98250.021.1497554
GGAACCG90100.021.061442
TCCCTTC793450.021.008218
CATCGGG204350.020.9742221
GGCGTAG97050.020.9578783
GGTAATC194000.020.8683261
TCGGGTG169150.020.8643041
GGGAGGA99700.020.8642275
TCGGGAG99050.020.8456633
Produced by FastQC (version 0.11.5)