FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56530503
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10499041.8572344916159687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACGT157700.022.0174054
CCGGGAA158750.021.5468041
CGGGAAC161800.021.0048082
ATACGAG53000.020.0962755
GTATTCA209300.018.1634989
GAACGTA214650.017.9109365
CCATTGT125150.017.905014145
GGGAACG217850.016.840463
TATTCAC210650.015.8994539
CGAGGGA109250.013.2242448
CCGTATA52100.013.180434145
CGTGTAT100800.013.051328145
TGGGCTA53750.012.8901052
TTTGATC104050.012.8268592
GTAGATA177400.012.549919145
TGTCCTA48300.012.3852655
GATCCTG74900.012.3697385
GTCTTTA76350.012.023711145
CACACGT118050.011.1824341
CCGATCT51600.011.1657712
Produced by FastQC (version 0.11.5)