FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_9424.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_9424.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14601642
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC212090.14525078754841408No Hit
CTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTCGCTACAAAATATA168060.11509664461024315No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG152850.10468000790596016No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC147280.10086536842911228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC104700.035.1137472
CGATCTA29800.034.5570834
CTCGGTT141500.034.181161
CCGGGAA265450.034.0917431
CTATTAG46950.033.0639538
TGTGCCG109050.031.188913145
AACGTAT352400.030.153226
TCAGATT61500.029.8305532
TACTACT40750.029.368946
ATTAGAA59500.028.6472456
TGGGCTA61800.028.2777232
GTATTCA383150.028.0011569
GGGAACG359850.027.326023
CCATTGT257500.027.092615145
CAATTAG60000.027.0815419
ACGTATT402450.026.8558587
CGTATTC404200.026.524968
CGGGAAC348300.026.336192
TATTCAC377550.026.2261649
TTGAACG70300.025.6926387
Produced by FastQC (version 0.11.5)