FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_9424.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_9424.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14601642
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC212090.14525078754841408No Hit
CTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTCGCTACAAAATATA168060.11509664461024315No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG152850.10468000790596016No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC147280.10086536842911228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC104250.034.7786032
CGATCTA29750.034.615114
CTCGGTT142450.034.3094831
CCGGGAA265100.034.2461131
CTATTAG47200.033.042358
TGTGCCG109300.031.183771145
AACGTAT352750.030.225966
TCAGATT61700.030.0865922
TACTACT40500.029.5500856
GTATTCA383050.028.1030129
ATTAGAA59300.027.8875266
TGGGCTA61750.027.713622
GGGAACG360350.027.3887753
CAATTAG60000.027.0814089
CCATTGT257450.026.928732145
ACGTATT402800.026.9224327
CGTATTC403800.026.6409248
CGGGAAC347850.026.474632
TATTCAC377900.026.2977249
TTGAACG70850.025.9025927
Produced by FastQC (version 0.11.5)