FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_9.21.2018.neg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_9.21.2018.neg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362677
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC528100.5096173508061672Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227320.21936416622847554No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATA118180.11404389039627502Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT58050.040.2238541
TCTAGTT71900.034.90867
GACATAC34100.034.6753279
TGTACTC68150.034.5807952
CCGTATA65850.032.70767145
AGTGTTA212800.031.215681145
CGTGTAA38900.027.031324145
CCGTGTT75250.026.598156145
AACAGAC47550.025.6250275
TAGTTGT96350.025.2973429
TACTCTA95950.025.1693084
GAGCCGG87900.023.677834145
AGACATA59950.022.6277778
TCTCGGG78900.022.24269145
CTCCAAC65400.021.8433151
CGTGTAT131750.021.796839145
TAGCATA36350.021.1539218
CTAGTTG119450.020.830398
AGCATTA59550.020.8250664
GCATTAG60600.020.8248635
Produced by FastQC (version 0.11.5)