FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_9.21.2018.neg_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_9.21.2018.neg_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362677
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATC528100.5096173508061672Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227320.21936416622847554No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATA118180.11404389039627502Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT58350.040.2650641
TCTAGTT72050.035.037337
TGTACTC68300.034.7168922
GACATAC34250.034.0999079
CCGTATA66050.032.718464145
AGTGTTA213050.031.51947145
CCGTGTT74900.027.01294145
CGTGTAA39300.026.571705145
AACAGAC47600.025.7503855
TAGTTGT96100.025.5141549
TACTCTA96300.025.3037854
GAGCCGG87400.023.647371145
AGACATA60350.022.2374218
CGTGTAT132100.022.178288145
TCTCGGG77950.021.86257145
CTCCAAC65450.021.826341
TAGCATA36650.021.178728
AGCATTA59950.020.9280824
TTACGAC42400.020.8688245
CTAGTTG119300.020.8566068
Produced by FastQC (version 0.11.5)