FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_8230.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_8230.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135582
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT394290.20605069655054128No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC301200.1574031038094373No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC270720.14147466222872135No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT247390.12928271531014837No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA243340.12716623931271076No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG224610.11737819105789413No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC222010.11601946572620578No Hit
CCCAAATCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGT221170.11558049292673721No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC217260.11353717906254432No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG216450.11311388386305678No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT209770.10962300493394975No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT193040.10088013001120111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAT91650.042.580957
TCCCTGA122800.039.44633
CCGTATA36500.036.95539145
CTCCCTG131200.036.6431772
AATCGAC130900.035.1280365
CCCAAAT106600.034.6260071
TCAGATT108250.033.3591422
TTGAACG114300.031.3506327
CCAAATC120350.030.908982
AAATCGA154050.030.1757834
ATCGACA131550.029.8839286
CCCTGAG164000.029.8042564
GAGTCTA65300.029.2049162
AAGAGTT127450.027.369251145
CAGATTG136600.027.3391323
GAAACTA68250.027.3081784
CTGAGCT188000.027.2380546
CCTGAGC184250.027.003555
GATCATG76050.026.8939235
AGTCTAA71100.026.6194523
Produced by FastQC (version 0.11.5)