FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_8230.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_8230.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19135582
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT394290.20605069655054128No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC301200.1574031038094373No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC270720.14147466222872135No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT247390.12928271531014837No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA243340.12716623931271076No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG224610.11737819105789413No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC222010.11601946572620578No Hit
CCCAAATCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGT221170.11558049292673721No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC217260.11353717906254432No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG216450.11311388386305678No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT209770.10962300493394975No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT193040.10088013001120111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAT91200.042.2338187
TCCCTGA122150.039.833853
CCGTATA36600.037.052914145
CTCCCTG131200.036.8087542
AATCGAC130200.034.9264765
CCCAAAT106800.034.425331
TCAGATT108100.032.8015172
TTGAACG114100.030.833077
CCAAATC120750.030.68632
AAATCGA153600.030.0276224
CCCTGAG164100.029.9181774
ATCGACA131850.029.4305976
GAGTCTA64450.029.0273592
GAAACTA69100.028.02134
CTGAGCT187050.027.4924836
AAGAGTT128000.027.42188145
GATCCTG62450.027.1757555
CCTGAGC184250.027.1606255
CAGATTG136600.026.914143
GATCATG76250.026.8230065
Produced by FastQC (version 0.11.5)