FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_8230.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_8230.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC687740.46199950920865235No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG510790.34313073153908097No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT296980.19950070411025325No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC267190.17948883133954666No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT228000.15316236964488433No Hit
GGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTT225950.15178525184763864No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC212510.1427567332159402No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA209160.14050632120580703No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC194290.13051717893993234No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG165120.11092180033229516No Hit
ATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATA163730.1099880472892847No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA157530.10582310565859046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA369950.050.5705871
CCGTATA33300.045.295338145
GGAACGT497150.039.458634
GAACGTA505600.039.419255
GGGAACG500050.039.385753
AACGTAT507750.039.2406856
ACGTATT517750.038.6262447
CGTATTC536650.036.9553688
CGGGAAC532750.035.5505372
GTATTCA565200.035.166719
CCATTGT328950.032.780495145
CGTGTAT106800.032.184113145
GTACGTT50500.032.165558145
CATTGTA258300.030.881805145
TATTCAC548400.027.8708639
CCGAGTC80450.027.1397767
CGTGTAA37300.025.856947145
CGTCGTT56750.024.4063145
GTAGATA223750.024.177435145
GTGGATG123700.023.271122
Produced by FastQC (version 0.11.5)