FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_8230.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_8230.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC687740.46199950920865235No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG510790.34313073153908097No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT296980.19950070411025325No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC267190.17948883133954666No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT228000.15316236964488433No Hit
GGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTT225950.15178525184763864No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC212510.1427567332159402No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA209160.14050632120580703No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC194290.13051717893993234No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG165120.11092180033229516No Hit
ATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATA163730.1099880472892847No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA157530.10582310565859046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA367350.050.553691
CCGTATA33050.044.541107145
GGAACGT494550.039.4023064
GGGAACG497100.039.3718073
GAACGTA503900.039.2933435
AACGTAT505350.039.1691446
ACGTATT515400.038.563547
CGTATTC533800.036.949398
CGGGAAC529200.035.5288542
GTATTCA562500.035.1424679
CCATTGT328950.032.670425145
GTACGTT50650.032.356792145
CGTGTAT106650.032.161533145
CATTGTA259100.030.898554145
TATTCAC546200.028.063139
CCGAGTC79900.026.6913977
CGTGTAA37750.025.933031145
GTAGATA223650.024.28563145
CGTCGTT56400.023.78642145
GTGGATG124200.023.6445982
Produced by FastQC (version 0.11.5)