FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_7651.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_7651.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14353270
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAG658540.45880834123513314No Hit
GTGCTTTTTAACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTA502360.3499968996611922No Hit
CCCGGATCAGCCCGCGAAGGACTTTAATGCAAGAAAATGGTCGATGAAGA474100.33030800646821246No Hit
CTCAAACTTCCATCGGCTTGAGCCGATAGTCCCTCTAAGAAGCCGGCGAC470200.32759085560293927No Hit
CCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTACCACCA360270.25100203646973823No Hit
GGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAAT356000.2480271046249391No Hit
CGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAGC305200.21263447284138043No Hit
CTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTG301310.20992428902960789No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA268340.18695391363779823No Hit
GCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCC259900.18107372048320697No Hit
GTTAGTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCCGCTTAG250820.17474763590457087No Hit
CTCGAACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAA245450.1710063281746947No Hit
CCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCA240850.16780148356437244No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT227800.15870947874595823No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT219700.1530661654103908No Hit
AAGGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTA216890.15110842337669397No Hit
GGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA207280.14441308496252073No Hit
CCGGGGCCGAAGCCTATTCGTATCCTCGTACGACGTGCTGGAGAAAATCG207000.1442180074645011No Hit
CCCGACTATCCCTATTAATCATTACGGCGGTCCTAGAAACCAACAAAATA192850.13435962676100988No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCG189820.1322486095502976No Hit
CCGGCACCTTAACCTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT183410.12778272825634854No Hit
AGACAGGTTAGTTTTACCCTACTGATGACCGTCGTCCCAATGGTAATTCC182540.1271765946017876No Hit
GCTCGAACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCA175020.1219373703692608No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA174660.12168655644323557No Hit
CCCCGTTACCCGTTGATACCATGGTAGGCCACTATCCTACCATCGAAAGT170650.11889276798945467No Hit
GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT168800.11760386309182508No Hit
CTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCCT161890.112789629122841No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC160120.111556460653217Illumina Single End PCR Primer 1 (96% over 33bp)
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC159210.11092245878465326No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT156910.10932003647949214No Hit
CCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGAACGGGTCAAAAT155720.10849095711290876No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATT154810.107856955244345No Hit
CGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAG154800.1078499881908443No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG153940.10725082158978406No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACATGC153430.10689550186124834No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAAC150520.10486808929254449No Hit
GCACGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACC148550.10349557975290649No Hit
CGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG146470.10204643262476078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTC38050.068.603072
TTGGTTC87700.058.123462
TCTCTTG47150.055.5299385
GAGTCTA112750.054.9851382
AGTCTAA114450.054.0454673
CTCGAAC88450.053.28541
GATCTCT49350.052.7513163
TGGCATC98900.050.1706059
ATCTCTT54450.048.347174
TAACATC227400.047.6063732
CTAACAT229400.047.3806421
TCGTGTT41650.046.833405145
TCGAACA85900.046.5108452
TAACTCT184700.046.4630139
TTAACTC184700.046.38438
GGAGTCT136050.045.781221
CATCTAT231900.044.5985265
ATCTATG231800.044.590676
TCTATGC232750.044.2253887
AGGAGTC81850.044.1170432
Produced by FastQC (version 0.11.5)