FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_6852.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_6852.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGAGAAAGAGAACCTGAAACCGTATGTCTACAAACAGACGAAGTCT12.0No Hit
CACGGTTAATTGCAGCCTCAACCTCCCAGGATCAAGCCATTCTCCCACCT12.0No Hit
AACGGAAGATACTAGCCGACAACAAGCACCCAAACACTACACTCGAAATA12.0No Hit
AACGGATTCGAAATACCAGTGTGTGAAGCTGAATGATGGTCACTTCATGC12.0No Hit
CCGGATTGATGGAAGCGCAGAAGAGCGCTGTCCCCGGGGACTTGCTCACG12.0No Hit
CCCTACGGTTACCTTGTTTCGACTTCACCCCAGTCATGAATCATACCGTG12.0No Hit
CAGGGACCCAAATAAAAGTGTTATGGAGGAAGAATACAAAGCCCCAACTG12.0No Hit
CTCTTGACAAAATATATATACTTTAAACGTCCTTTCATCTCCCTTCACAA12.0No Hit
CTAGCGTCAGCAGCGCCACACATACCGTAAAATGAAGCTTAGACATCCTA12.0No Hit
CGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG12.0No Hit
ACTAAGTATTTTTTTCACAGTTTTCCGGGCTTTTCTTAAACTGCCAAGAT12.0No Hit
GCGAGCCACGCCCCACCCTCGGCCATCCCGTTGTAGGTGGCCATGAGGAC12.0No Hit
TGTCGATAGGGCAGAAACGGTCGATAGTGGCGATACGGTCGCTCCTGACG12.0No Hit
TGGAAAGCAGCCAGAACCCCTGCCACTGGATTCTTCAGCACCCTTGGGAT12.0No Hit
CTCCGACAACAACCCAGCGTTTCCCAAACAGAACCGCCACTAACGCAAGC12.0No Hit
CAGCAAACTTGCGTGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCA12.0No Hit
GAAATGGCCATTTTGTAGGACAAAACCAAGCATTGGCAATTTCACATGGT12.0No Hit
GGATCTTCATCTCAACATCCAAACACACAAATGGAATGCAGTAACACCTT12.0No Hit
GTCTGTCTTAAAAGTTCGGGGCTTAACCCCGTGATGGGATGGAAACTGCT12.0No Hit
GGAGGGCGCACCCTTTCATGGCTCGATTCATGGCGGAATTAGCGACACAA12.0No Hit
CTGTGTGTGACCTTTACAGACTTCCAATGGCTTTGCCTGGCCAGAGGGCA12.0No Hit
GACGAAAAAAACCACAACCTCAACAACATCTGCTTGGAACCCTCAGTACC12.0No Hit
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG12.0No Hit
CCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCATGGAGTCG12.0No Hit
AAAAAGCAACAAAACCAGCTTAGGATAACACTTTTTGTTGCTTGTTCTTA12.0No Hit
CAGCTCCAAACGCTTATAATAAAGAATCTTCACTTAAAAAGATCTTAGAA12.0No Hit
GCCGAGTTCCTTAACCATGATTATCTCGTGCGCCTAGGCATACTCTGCCC12.0No Hit
GGCTTCCTAGCTTGGGCAGCTTTAAACCACTGAAAAGAAGAAAAGGCTGC12.0No Hit
CGCTGCCTACCGTAGGAGTCTGGGAAGTGTCTCAGTCCCAGTGTGGCTGG12.0No Hit
AGAACAACGCATGCCCCCTACTTAAAACCTACGAAACACTCCCCAACTTC12.0No Hit
CGGAGATTGGTCGTATCCGGTATTAGACCCAGTTTCCCAGGCTTATCCCA12.0No Hit
GCGCTTTAGCGTCAGTATCTGTCCAGTAAGCTGGCTTCCCCATCGGCATT12.0No Hit
GGTGGATGCCTTGGAAATCAGAGGCGATGAAGGACGTGCTAATCTGCGAA12.0No Hit
CTAATGTACCCGAAGATAGGCAGGCATGATGCCGAGATCGTTGCCGAGGG12.0No Hit
GTGTCACTTCTCCGTCTCCACATCGCCCCAAGGGATAGTAAAGGAATATT12.0No Hit
GAACAGTCGGACAGCTCTGTGACACACGAGGACACTGAGGCCCGGAGAAA12.0No Hit
CAATAAATCAAAATAAAACTAATAGAATAAAAAACTAGTTATAGTCAACA12.0No Hit
CCTGGGCCAGGCCTGGTGCCTGAGTGTGAAGACTCCTGGCCTGCGTGGCA12.0No Hit
TGCCTTCTGGCTGCAAGAAATGCAACCCTCTTTTACCACCAAGGAGGCCC12.0No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT12.0No Hit
CAACCAATCAATGCTGAAAATATAATCTGAGCTATGTAGCCACGTAGAAA12.0No Hit
TGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAG12.0No Hit
CCGCTCTAATTTTCGTACGCTTAGTACCACTATTTACATTTCGAATACGG12.0No Hit
TGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTA12.0No Hit
AACAAAACAAAATTACACTTAAGCACTATTGTTTAATTTTTAATTGTCAG12.0No Hit
CTCCACCACGGACAACGAGAAGCTCCACCTAAAAGCATTTCGGGTAGAAC12.0No Hit
ACTCCATGAGCCCCCCTTAGGACCCCTGCGTTATGGTTTAACAGATGTGC12.0No Hit
GCTGGCGTCGTAGTGCGCGACGAGGGTGGCGTCCAGGCCCGCGGCGGCCA12.0No Hit
GCAGAATTAGAGCAACTCAAATGGATATAAAGATGGAGGGGAGAGGAGAT12.0No Hit
ACTATATTAGTGACTGTAAGGAGCTGCTTAGCTATTGAAGAGCTTCTCTC12.0No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA50.0145.0145
GATACTA50.0145.08
GAAGATA50.0145.05
CGGAAGA50.0145.03
AAGATAC50.0145.06
GGAAGAT50.0145.04
ATACTAG50.0145.09
AACGGAA50.0145.01
ACGGAAG50.0145.02
AGATACT50.0145.07
Produced by FastQC (version 0.11.5)