FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_4329.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_4329.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15599940
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG157200.028.830221145
TCGTGTT112000.025.056099145
CTCTATT124600.024.79203145
AACGTAT171150.021.9512025
CTATGTT76900.021.122349145
TGTCCTA88650.019.635325
GAACGTA204150.019.5030884
CGATCTA37350.019.0289964
CGGGAAC189450.018.8322471
GAGTCTA50850.018.681152
TGTGTAT87700.018.107773145
ACGTATT212250.018.0092756
CGTATTC208350.017.9995238
TATTCAC209100.017.7267639
GGAACGT191900.017.6868824
GTATTCA218150.017.1244248
CCGGGAA212400.016.3194331
GTAGATA184750.015.778369145
CACACGT115650.015.6756671
ATGCCGT104400.015.4941718
Produced by FastQC (version 0.11.5)