FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_4329.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_4329.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15599940
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG156650.028.422342145
TCGTGTT112750.025.404016145
CTCTATT125000.024.886805145
AACGTAT170900.021.7722366
CTATGTT76850.021.0418145
TGTCCTA88650.019.5532535
GAACGTA204350.019.4127064
CGATCTA37150.019.131134
CGGGAAC188950.018.80521
GAGTCTA50750.018.717722
CGTATTC207400.018.0817388
ACGTATT212000.017.8934296
TGTGTAT87350.017.848322145
GGAACGT192050.017.6350214
TATTCAC208750.017.5825149
GTATTCA217650.017.1634869
CCGGGAA212900.016.1788181
CACACGT116600.015.7966141
GTAGATA185750.015.6153965145
ATGCCGT104150.015.2525768
Produced by FastQC (version 0.11.5)