FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30981.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30981.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495416
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC179850.11606658382066026Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA23400.036.879856145
TCGTGTT84350.031.037035145
GTAGATA150750.020.156487145
CGTCGTT24600.018.27741145
GAGCGTG49700.018.093534145
GGGTGTT152800.017.655405145
ATAGGGG113400.016.499271145
TCTCGGG76950.016.398296145
CGGTGGG95900.014.897237145
AGAGCGG127450.014.794205145
TTACGAC12950.014.5612795
TCGTGCG21650.014.4068237
CAACCGA35750.014.4027234
TGTAGGT140350.013.847791145
CGTGCGT22100.013.7852968
CGGGGTT123500.013.6816991
TCGGAAA35200.013.391487145
AGCTCGC59400.013.3107329
GTTACGA14350.013.1396494
TACGGAC38750.012.727215
Produced by FastQC (version 0.11.5)