FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30981.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30981.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495416
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC179850.11606658382066026Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA23150.036.651302145
TCGTGTT84650.031.098125145
GTAGATA150700.020.547981145
GAGCGTG49650.018.549791145
CGTCGTT24650.018.240189145
GGGTGTT153000.017.632181145
TCTCGGG76400.016.706055145
ATAGGGG113800.016.696045145
AGAGCGG127400.014.799889145
CGGTGGG96050.014.798348145
TTACGAC12900.014.6178365
TGTAGGT139750.014.114699145
CAACCGA35600.014.056014
TCGTGCG21750.014.007157
AGCTCGC59450.013.66568859
CGTGCGT21900.013.5800348
CGGGGTT124400.013.4079821
TACGGAC38850.013.2546035
GTTACGA14250.013.2318774
TCGGAAA35300.013.148004145
Produced by FastQC (version 0.11.5)