FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30981.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30981.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940757
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC244820.1756145666982073No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG180060.12916084829539745No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT165900.11900358065204063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA50650.039.800888145
AACGTAT263600.030.351976
AGTGTTA260700.027.871101145
ACGTATT292050.027.8455037
GAACGTA290800.027.6851185
GTATTCA293050.027.4044029
CGTATTC296200.027.3087088
CGTGTAT133850.027.25066145
CCGGGAA277600.026.5389981
TCAGATT79900.026.4094622
GATCCTG42250.025.7503015
GGAACGT305400.025.5041564
GATCATG44400.025.3201665
GGCCAAC72950.025.2475171
GGGAAAA136600.024.57863145
GAAACTA67350.024.1204914
TCCCTGA72650.023.7566033
CCGTGTT88550.023.42097145
GGTTAGA91300.023.1152134
CCATTGT194600.022.730783145
Produced by FastQC (version 0.11.5)