FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30981.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30981.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940757
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC244820.1756145666982073No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG180060.12916084829539745No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT165900.11900358065204063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA50400.039.998463145
AACGTAT262650.030.3236716
AGTGTTA259900.027.901192145
ACGTATT291700.027.7543727
GAACGTA290250.027.6126385
GTATTCA292600.027.322399
CGTATTC295750.027.2274238
CGTGTAT133500.026.779022145
TCAGATT80100.026.6152062
CCGGGAA276900.026.5015341
GATCCTG42000.026.2489645
GGAACGT304750.025.4157734
GGCCAAC72250.025.3919541
GGGAAAA135750.024.786041145
GATCATG45200.024.711565
GAAACTA67650.024.2279384
CCGTGTT88950.023.723354145
TCCCTGA72700.023.6406043
GGTTAGA91550.023.3689634
TTGAACG92200.022.6602767
Produced by FastQC (version 0.11.5)