FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30979.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30979.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16749640
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG395350.2360349237356743No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC322610.1926071246904411No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT284490.16984842659304916No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG272040.1624154310182189No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC269050.1606303180247456No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC263120.15708994342564975No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG256370.153060006065802No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC249490.1489524550975424No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC236070.1409403425984081No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA235840.14080302621429475No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG228410.13636710997967716No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA224740.13417601811143404No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG216270.1291191930095214No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT211190.12608629200388785No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC201520.12031303359355784No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT200810.11988914388607755No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA186980.1116322500065673No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA184530.11016953200188183No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA180410.10770977764298219No Hit
CTGGGCTACACACGTACTACAATGGGAGTTAATAGACGGAAGCAATACCG176280.10524405300651238No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT172960.10326192085322432No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC171760.10254548754480694No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT171250.10224100338872955No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG168760.1007544042737635No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC168450.10056932566908901No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC167820.10019319818216987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTA58450.053.096452
TCGATCT16150.048.9424933
GAAACTA95500.046.9294854
TCCCTGA115200.046.6442223
CTCCCTG114950.046.6167142
GATCATG29500.041.304495
CCATTGT139150.041.27315145
GATCCTG27750.040.2501685
AGAAACT114750.038.864633
CTAACCA116950.037.8997738
CGCTCAG42450.036.9008834
AGTGTTA149100.036.865253145
AAGAAAC121550.036.807512
CCGTATA22750.036.655746145
ATCGACC61350.036.0607036
CATTGTA96300.035.767864145
GAGTCTA54200.035.1854132
CGTGCGT56700.034.0325288
TTGAACG49300.033.8430947
TCAGATT50050.033.4667932
Produced by FastQC (version 0.11.5)