FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13796210
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT266970.1935096667852983No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT200640.14543124524778905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA36150.062.384777145
TTCGCCG105050.030.786856145
GTAGATA209800.029.033562145
GAGTGTA168750.027.415958145
GATCCCG159050.026.0342313
ATCCCGA163450.025.824154
TCGTGTT79800.024.262463145
TGTGTAT116100.022.422745145
CGGTGGG63900.022.242403145
AACGTAT92100.021.8952716
CGTCGTT53800.021.430948145
GGGAAAA214650.021.283163145
TACGGAC109450.020.8748665
AAGAGTT187250.020.563604145
TGGGATC159550.020.45262
GAACGTA98900.020.2414535
TCCCGAG213450.019.9466925
ACGTATT101650.019.9110187
GCCTGTT123100.019.085938145
CGTGCGT32450.019.0024158
Produced by FastQC (version 0.11.5)