FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30978.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30978.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13796210
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT266970.1935096667852983No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT200640.14543124524778905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA36300.062.327084145
TTCGCCG105050.030.85605145
GTAGATA208900.028.881102145
GAGTGTA168250.027.799278145
GATCCCG158700.025.8634053
ATCCCGA163300.025.5372144
TCGTGTT79950.023.76357145
TGTGTAT117050.022.240875145
AACGTAT92500.022.0359656
CGGTGGG63600.021.891373145
CGTCGTT53900.021.391302145
GGGAAAA214750.021.10453145
TACGGAC109500.020.8655225
AAGAGTT187350.020.552738145
GAACGTA99400.020.4316985
TGGGATC159250.020.4001672
ACGTATT102000.020.0562387
TCCCGAG213650.019.7245035
CGTGCGT32000.019.2698088
GCCTGTT123300.018.996271145
Produced by FastQC (version 0.11.5)