FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30908.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30908.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072756
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC273510.181459847157348No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG228180.15138571870996917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA42350.045.20794
TCGATCT51250.035.6572153
GATCCTG107900.034.482385
CTATTAG82550.031.4586988
CCGGGAA334400.030.294091
CTCGATC62050.028.2809622
CTGGCTC130550.027.393159
AACGTAT385000.027.0350446
TATTAGT105350.026.9224269
GGGAACG412600.025.8010643
GGAACGT347700.025.737974
TGTCCTA62750.025.659055
ATCTATT91550.025.115216
TGGGCTA164150.025.003232
CGTATTC422300.024.6837148
GTATTCA427400.024.3890119
CGGGAAC425750.024.1684232
TGATCCT143450.023.406924
CAAGTTA78200.022.5372475
GAACGTA467400.022.329215
Produced by FastQC (version 0.11.5)