FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30908.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30908.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072756
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC273510.181459847157348No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG228180.15138571870996917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA42550.045.677144
TCGATCT51600.036.118153
GATCCTG108050.034.2330135
CTATTAG82900.031.850788
CCGGGAA335400.030.3117581
CTCGATC61750.029.0055052
TATTAGT105450.027.3095329
CTGGCTC130050.027.2752579
AACGTAT386200.027.0824096
TGTCCTA62600.026.068015
GGGAACG414250.025.8034043
GGAACGT348900.025.6702254
ATCTATT91900.025.2562436
TGGGCTA163850.025.0047452
CGTATTC424400.024.7324078
GTATTCA429350.024.3964189
CGGGAAC427400.024.1429752
TGATCCT142750.023.3692844
CAAGTTA78200.022.5371635
GAACGTA468250.022.3815295
Produced by FastQC (version 0.11.5)