FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30907.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30907.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308158
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC177920.14455453041795532No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG152740.12409655449661923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA76350.039.8986135
GAGTGTG105050.038.103848145
TGTGTAT67900.033.00004145
AAGAGTT98450.029.09425145
GTAGATA217250.028.905725145
GTGTCCT107700.028.8210834
CTACTCT111750.028.3668779
GATCCTG99050.028.3383165
GGAACGT197650.027.740054
AACGTAT305850.026.8222146
CCTACTC121900.026.2431558
CGATCTA30250.026.1326454
GTCCTAC119900.026.0734566
GTATTCA323600.025.4203859
CGTATTC325850.025.3563738
TCGTGTT62150.025.202196145
GGGAAAA142100.025.056084145
CCGGGAA225150.024.7677881
CGGTGTC119400.024.7185462
ATCTATT35750.023.941236
Produced by FastQC (version 0.11.5)