FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30907.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30907.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308158
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC177920.14455453041795532No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG152740.12409655449661923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA77000.039.8449675
GAGTGTG104650.038.11068145
TGTGTAT67650.032.585846145
GTGTCCT107800.028.9290854
AAGAGTT99400.028.888968145
GTAGATA218200.028.713242145
CTACTCT111900.028.459039
GATCCTG98950.028.2207645
GGAACGT198400.027.5988064
AACGTAT306150.026.6542726
CGATCTA30550.026.350984
GTCCTAC119500.026.3433136
CCTACTC122300.026.2764348
GTATTCA324250.025.2580389
GGGAAAA141700.025.229015145
CGTATTC326200.025.2184438
TCGTGTT62300.025.14137145
CGGTGTC120050.024.8263822
CCGGGAA226100.024.5995311
ATCTATT35850.024.279556
Produced by FastQC (version 0.11.5)