FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30905.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30905.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803480
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC219380.22377767894665976No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG161470.1647068183951005No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA125010.12751594331808705No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC106220.10834928005157352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA20800.048.46584
GATCATG39750.039.4136855
TCGATCT26450.037.2879333
TGTCCTA41950.036.4821935
CTATTAG40250.034.6065988
GATCCTG46000.034.5316055
TTGATCA44300.034.049813
CCGGGAA190350.033.4843831
TTTGATC91400.033.401552
TATTAGT46800.032.7087979
TCAGATT66250.032.5088272
CAGATTG70150.031.2201043
AACGTAT259950.029.4962356
CTCGATC35200.029.0473942
TTGAACG74050.028.5096267
GGAACGT235000.028.4233784
CCATTGT182750.027.49624145
CGTATTC280300.027.4609688
GGGAACG245350.027.3703293
GAACGTA288100.026.8376455
Produced by FastQC (version 0.11.5)