FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30905.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30905.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9803480
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC219380.22377767894665976No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG161470.1647068183951005No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA125010.12751594331808705No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC106220.10834928005157352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA20700.048.7001154
GATCATG39750.039.231365
TCGATCT26150.037.4385263
TGTCCTA42150.036.8254665
CTATTAG40100.034.5550848
GATCCTG46200.034.2252435
TTGATCA44250.033.9245263
CCGGGAA190750.033.566521
TTTGATC91400.033.2429962
TATTAGT46650.032.658419
TCAGATT66150.032.338842
CAGATTG70300.031.0504473
AACGTAT259550.029.59776
CTCGATC34850.029.1311452
GGAACGT234900.028.4973344
TTGAACG74700.028.3586257
CCATTGT182850.027.600128145
GGGAACG245400.027.5421623
CGTATTC280100.027.506448
GAACGTA287800.026.9161245
Produced by FastQC (version 0.11.5)