FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30905.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30905.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14653360
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG61400.035.441728
GCAATTA52050.032.3317458
TTCTGGC82600.027.223387
CGAGCTA43500.025.8400344
TTGATTC89200.024.2249853
TTTGATC91100.024.1164912
TATTAGT97450.023.5220979
CGATCTA15150.023.4549274
CAATTAG72900.023.0850739
GATCCTG101600.022.4145285
TCTGGCT112000.020.9192648
ATTCTGG108200.020.7806556
AGCAATT83050.019.7391857
CTGGCTC217450.019.6483759
GAGTGTT58000.017.87909145
GGGAAAA86000.017.623217145
CGACCTA22850.017.4552944
GTTTGAT282050.017.2765981
TGGATGC140050.017.1378773
TGGTTCG66750.017.0553463