FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30905.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30905.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14653360
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG60700.035.9703258
GCAATTA51850.033.0164078
TTCTGGC82200.027.6208887
CGAGCTA43250.025.9895924
TTGATTC88800.024.4973553
TTTGATC90750.024.1294542
TATTAGT97200.023.657489
CAATTAG72500.023.6129079
CGATCTA15300.023.2251514
GATCCTG101500.022.3653225
TCTGGCT111250.021.060528
ATTCTGG107800.020.858066
AGCAATT84050.019.8497567
CTGGCTC217650.019.6305339
GAGTGTT57950.018.019724145
CGACCTA22800.017.4937024
GGGAAAA86850.017.450808145
GTTTGAT282300.017.2355631
TGGATGC139950.017.2018873
GGATGCC158100.016.6046834
Produced by FastQC (version 0.11.5)