FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30904.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30904.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016551
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT385880.27530310416592496No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT288020.20548564336547556No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC245820.17537837946011112No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC193950.13837212877832783No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG170960.12197009093035797No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA156760.11183921065888462No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT148230.10575354807327424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTTG65700.047.13223145
TGTGTAT35400.043.429825145
AACGTAT243900.033.038226
GAGTCTA49200.033.011872
CCGTATA26050.032.571186145
GAACGTA259550.031.1287365
GATCCTG44900.030.8520745
CGTATTC264250.030.77178
TTCGCCG130550.030.441015145
TCGTGTT75100.030.127987145
CGGGAAC263850.030.0365682
AAGAGTT92000.029.95375145
ACGTATT279200.029.2796787
GTATTCA283850.028.621559
TTACTTC54050.027.9078394
GGAACGT285500.027.5855924
GAAACTA106550.026.6803284
TACTTCC56250.026.4319155
TGGATGC56700.025.9608483
TCGATCT20900.025.3269233