FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30904.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30904.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016551
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT385880.27530310416592496No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT288020.20548564336547556No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC245820.17537837946011112No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC193950.13837212877832783No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG170960.12197009093035797No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA156760.11183921065888462No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT148230.10575354807327424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTTG65150.047.1965145
TGTGTAT35200.042.852783145
AACGTAT244550.033.0986826
CCGTATA25850.032.82341145
GAGTCTA49000.032.7026752
GAACGTA259800.031.2104345
CGTATTC264450.030.8033878
TTCGCCG131150.030.689022145
GATCCTG44650.030.375075
TCGTGTT75400.030.296858145
CGGGAAC264400.030.083772
AAGAGTT92000.029.7963145
ACGTATT279300.029.34727
GTATTCA284450.028.6376679
TTACTTC54600.028.0251664
GGAACGT285800.027.6834954
GAAACTA106250.027.0286674
TACTTCC56650.026.7573765
GTCCTAC87900.025.3316636
TGGATGC56300.025.2438093
Produced by FastQC (version 0.11.5)