FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30903.01.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30903.01.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15803871
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT101600.041.89713145
TGTCCTA68800.030.888645
CTATTAG62700.029.1555568
AGTGTAT98600.027.432903145
CTGGCTC166550.025.2186769
TATTAGT89450.022.950629
GATCCTG204000.022.7901635
TGATCCT200050.022.5488644
TAGTGTT57550.020.539133145
CTACTCT106200.019.9455819
GTGTCCT112100.019.7318154
CGATCTA57850.019.5566334
TTTGATC216600.019.215822
CCTACTC121000.018.4052358
CCTGGCT215350.018.3923038
TCCTGGC223500.018.272937
AACGTAT271750.018.2293325
TTGATCC226050.018.1251853
TATAGTT142700.017.83705145
ATCTATT72300.017.1558886
Produced by FastQC (version 0.11.5)