FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30900.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30900.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335039
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA191930.11071795108162144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT85450.034.96523145
AGTGTAT87750.020.743298145
CTGGCTC182600.020.6579849
GATCCTG266700.020.3150425
TCGTGTT67250.017.684908145
TGATCCT296950.017.6334154
GTTTGAT265800.016.3957231
CCGTATA30900.016.19354145
CCTGGCT255850.014.2899018
GTAGATA141200.014.2778015145
AGTACTA92350.014.0604838
CAATTAG94850.013.9148253
TAGTACT95000.013.6679147
TCCTGGC272750.013.4307257
CGTGTAT62700.013.185255145
TTTGATC311100.013.0994682
TAGTGTT80700.013.030039145
TTGATCC300600.012.9306253
CTATTAG58350.012.9293478
AATTAGT112350.012.8458424
Produced by FastQC (version 0.11.5)