FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30900.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30900.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335039
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA191930.11071795108162144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT84500.034.757336145
AGTGTAT87400.020.74325145
CTGGCTC181650.020.5663269
GATCCTG265450.020.2740655
TCGTGTT67200.017.697943145
TGATCCT295750.017.6314264
GTTTGAT264000.016.260391
CCGTATA31100.016.089293145
AGTACTA92750.014.31267458
CCTGGCT255350.014.2894588
GTAGATA140950.014.200128145
CAATTAG95650.014.101693
TAGTACT94900.013.988057
TCCTGGC272900.013.3435857
AATTAGT113100.013.1454244
TAGTGTT81200.013.039023145
TTTGATC310200.013.02061752
CGTGTAT63150.012.976373145
ATTAGTA126700.012.9375335
TTGATCC299500.012.8328253
Produced by FastQC (version 0.11.5)