FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30900.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30900.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18014631
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC483780.2685483815904972No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG333750.18526607622437563No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA185010.10269985546748085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA66100.044.7686235
AGATTAT217100.042.922714145
CCGGGAA446500.041.7688371
GATCATG46200.040.032555
ACGAGCA79650.039.972054
TCGATCT25600.038.5246963
GAGCAAT90850.037.764516
CGATCTA28000.037.2977074
AGTGTAT101800.036.04513145
GATCCTG57100.034.5499845
GTGTCCT85650.034.547014
AGCAATT99700.034.270127
AACGTAT611550.034.218546
GGGAACG589200.033.64933
CTATTAG55650.033.6318248
CAATTAG119350.032.518899
GTATTCA659000.031.7148159
GCAATTA123050.031.6583738
GGAACGT633050.031.2066214
TTTGATC121750.031.0894362
Produced by FastQC (version 0.11.5)