FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30900.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30900.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18014631
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC483780.2685483815904972No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG333750.18526607622437563No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA185010.10269985546748085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA66300.044.3054475
AGATTAT217600.042.890675145
CCGGGAA446600.041.857071
ACGAGCA78900.040.3520624
GATCATG46300.039.6328355
TCGATCT25900.038.3586043
GAGCAAT90500.037.9907576
CGATCTA28000.037.8157774
AGTGTAT101800.036.543724145
AGCAATT98900.034.620727
AACGTAT613200.034.2329676
GTGTCCT86250.034.054474
CTATTAG55850.034.0309838
GATCCTG57550.034.027815
GGGAACG590300.033.6604423
CAATTAG118800.032.6084189
GCAATTA122700.031.7487168
GTATTCA660150.031.7475199
GGAACGT634700.031.2169424
TTTGATC121400.030.9400162
Produced by FastQC (version 0.11.5)