FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30893.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30893.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805174
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA37950.027.3275244
CCGGGAA156850.023.3014071
CTATTAG78600.023.0728958
TCGTGTT76100.020.488145
GGAACGT181950.020.4475544
CGGGAAC180300.019.9886952
GGGAACG193350.019.577863
AACGTAT212850.019.0839126
CGAGCTA35650.018.9190544
GATCCTG122850.018.7156165
CGTGTAT92150.016.289988145
GATCTAT75450.015.8615185
GAACGTA271500.015.7066484
TCGATCT59550.015.5871633
GTACGTT40600.015.360954145
CCGTATA26600.015.266947145
CGTATTC274700.015.0522298
GTATTCA276100.014.9761439
TATTAGT129150.014.4354479
GGTTAGA132300.014.4169024
Produced by FastQC (version 0.11.5)