FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30893.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30893.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805174
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA38050.028.2087544
CTATTAG79450.023.8307428
CCGGGAA156500.023.0756911
GGAACGT181650.020.2018874
TCGTGTT76500.020.190939145
CGGGAAC179400.019.8464872
GGGAACG192850.019.3278333
GATCCTG122600.019.0496755
CGAGCTA35850.019.015844
AACGTAT211900.018.9641786
CGTGTAT92100.016.849712145
GATCTAT75450.016.6306445
TCGATCT59850.016.2360483
GTACGTT40600.015.896528145
GAACGTA271200.015.75079254
CCGTATA26550.015.295434145
CGTATTC274150.014.9238818
TATTAGT130300.014.8649929
GTATTCA275500.014.85098659
GGTTAGA131950.014.34524254
Produced by FastQC (version 0.11.5)