FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30891.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30891.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559836
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA27550.043.1681064
GATCCTG90050.036.644875
TGTCCTA58500.035.7044335
GCAATTA82450.034.6642768
CAATTAG86650.033.1510439
CTGGCTC107400.031.6091029
CTATTAG64950.031.16038
CTCTATT76250.030.529266145
TGTGCCG148700.030.382835145
TGATCCT108150.029.9053634
AGCAATT78000.029.7596247
GAGCAAT84650.029.73276
ACGAGCA78100.028.226914
GTGTCCT75400.028.0835654
TTTGATC139500.027.392172
TCACGAG84150.026.9699572
CTATGTT46200.026.684444145
TCGATCT43250.026.6582053
CCGGGAA326350.026.241441
CGAGCAA92100.025.7497885
Produced by FastQC (version 0.11.5)