FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30891.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30891.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559836
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA27550.043.1687664
GATCCTG89350.036.283165
TGTCCTA58300.035.205465
GCAATTA82400.034.861678
CAATTAG86700.033.2995389
CTATTAG64700.031.1688448
CTGGCTC106900.031.14659
CTCTATT75500.030.832266145
TGTGCCG148750.029.933588145
AGCAATT78300.029.831147
GAGCAAT84450.029.8035666
TGATCCT107800.029.3974784
ACGAGCA77700.028.3726524
GTGTCCT75350.027.5251794
TCACGAG84100.027.1586552
TTTGATC139700.026.9898572
TCGATCT42850.026.7381443
CTATGTT46400.026.725481145
CCGGGAA325600.026.4132771
CGAGCAA92100.025.8289265
Produced by FastQC (version 0.11.5)