FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30830.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30830.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981437
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC232300.17894783143037246No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG221740.17081313879195348No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC171390.13202698591843107No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG143540.1105732747460855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG65200.043.71875
CGATCTA35750.038.137944
TCGATCT34150.037.584993
ACGTCCG67550.032.742218145
CCGGGAA264900.030.3023131
GTTAGAG107150.029.0393855
TCGTGTT61550.028.629374145
CTATTAG94750.027.4092068
TTAGAGT116050.027.3774596
CTCGATC47750.027.3348052
AACGTAT338550.026.868226
CTGGCTC114750.026.3624679
TGATCCT105750.026.1288364
CATTGTA158100.025.73147145
GGAACGT327400.025.1637344
TGTACGG61800.024.876026145
TTTGATC124800.024.6358812
GTATTCA377400.024.104519
GATCCCG61050.023.9937213
GAACGTA380400.023.9290545
Produced by FastQC (version 0.11.5)