FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30830.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30830.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981437
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC232300.17894783143037246No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG221740.17081313879195348No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC171390.13202698591843107No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG143540.1105732747460855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG64950.043.4407125
CGATCTA36100.038.170194
TCGATCT34200.037.954183
ACGTCCG67100.032.5292145
CCGGGAA264200.030.0807531
GTTAGAG106850.029.1211995
TCGTGTT61200.028.674335145
CTCGATC47600.027.725782
CTATTAG94900.027.5188148
TTAGAGT115300.027.4298746
AACGTAT337950.026.7228986
TGATCCT105750.025.9918234
CTGGCTC115400.025.8997129
CATTGTA157800.025.642279145
GGAACGT327100.025.0095864
TTTGATC124150.024.5897872
TGTACGG60550.024.431234145
GATCCCG60700.024.2515833
GTATTCA376900.023.9633059
GAACGTA380700.023.7770635
Produced by FastQC (version 0.11.5)