FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30786.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30786.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14991560
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC310560.2071565600911446No Hit
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC268170.1788806501791675No Hit
CACACGTGTTACAATGGGGGGTACAGAAGGCCGCTACCCGGCGACGGGAT160790.10725368140473705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG90650.046.0862355
TGATCCT92350.043.58454
CTGGCTC107750.038.1726159
CCTGGCT115350.035.6569638
TTTGATC137750.034.848992
TCCTGGC120900.033.7793167
ATCCTGG151200.028.5915136
TTGATCC149100.027.917433
CCGGGAA393200.026.1134011
AACGTAT509850.024.2975255
TCGTGTT89500.023.091768145
TTGAACG75850.023.047847
CGATCTA20450.022.6966974
TGTACGG71650.022.670822145
GTATTCA545600.022.4693898
ACGTATT561850.022.2953686
CGTATTC549600.022.2525427
GAACGTA565150.022.1361144
GGGAAAA133300.022.086725145
GGCTCAG113300.022.083364
Produced by FastQC (version 0.11.5)