FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30786.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30786.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14991560
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC310560.2071565600911446No Hit
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC268170.1788806501791675No Hit
CACACGTGTTACAATGGGGGGTACAGAAGGCCGCTACCCGGCGACGGGAT160790.10725368140473705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG91200.046.5236135
TGATCCT92450.044.2430954
CTGGCTC108450.038.5948839
CCTGGCT116200.036.020188
TTTGATC137750.035.5858732
TCCTGGC121600.034.1215327
ATCCTGG152050.028.860746
TTGATCC149150.028.2969533
CCGGGAA392550.026.2304521
AACGTAT509850.024.368425
TGTACGG70850.023.131674145
TCGTGTT89500.022.848864145
GTATTCA543950.022.5774128
TTGAACG76150.022.575877
CGATCTA20300.022.507014
GGGAAAA132750.022.396896145
ACGTATT561350.022.3666976
CGTATTC548900.022.3336437
GAACGTA564550.022.2494244
CGGGAAC498750.022.1135641
Produced by FastQC (version 0.11.5)