FastQCFastQC Report
Sat 10 Nov 2018
HVCHNBGX7_n02_MT_30783.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHNBGX7_n02_MT_30783.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11093977
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC458180.4129988731723529No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG334380.30140679036922463No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA195290.17603245436690557No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC146820.13234208075246595No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA141080.12716810211522883No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT130370.1175142151457498No Hit
CGGCGAGCGAACGCGAAGGAGCCTGTTAGTGATAATGACAGAGACAGAGG116410.10493081065518706No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGATAGTGCGAGC112760.1016407371315084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA232450.040.3653141
GAGCAAT78300.034.8301286
GGTTAGA93950.034.4257054
AACGTAT301200.033.5688746
GCAATTA92400.033.3639378
ACGAGCA77950.032.9330444
GGGAACG303400.032.2890433
TCACGAG78650.031.8992
TGTCCTA51000.031.14385
ACGTATT333200.030.6956217
CAATTAG101500.030.5157559
AGCAATT93850.030.452597
GAACGTA346950.029.8928135
GGAACGT337900.029.402024
GTATTCA346500.029.2033929
CGAGCAA96700.028.3506475
CCATTGT222950.028.264761145
CGTATTC394350.025.678168
CGGGAAC389500.024.6106852
TATTCAC319750.024.2509889